The HaloTag Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications. The HaloTag Iodoacetamide (O2) Ligand contains a reactive iodoacetamide group connected to an alkylchloride separated by an ethylene glycol repeat (O2). HaloTag Iodoacetamide (O2) Ligand has been designed to rapidly react with sulfhydryl-containing molecules (see figure), whether small organic compounds, peptides or proteins. The ligand with functional group can then be used with the HaloTag protein for any application of interest.
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